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Fig. 5 Competition of EPI-X4, WSC02, JM#21 and AMD3100 with the CXCR4 ECL-2 antibody 12G5. CXCR4 expressing SupT1 cells were precooled and assay buffer was removed. EPI-X4 and the optimized derivatives WSC02 and JM#21, and the small molecule AMD3100 were diluted in cold PBS and added to the cells. Immediately afterwards, a constant concentration of the APC-labelled CXCR4 antibody (clone 12G5) was added. After 2 h incubation at 4 °C unbound antibody was removed and cells analyzed in flow cytometry. Data were represented as mean ± SEM (n = 3). IC50 values were calculated by non-linear regression.
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