ZFIN Image: Deng et al., 2022, Figure 3.

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Figure Caption

Figure 3.

Overproliferation of macrophage progenitor is the main cause of macrophage lineage expansion in zbtb14 mutants.

(A–C’, E–G’) pH3 and transferase dUTP nick end labeling (TUNEL) assays in zbtb14^-/-//Tg(mpeg1.1:eGFP) and Tg(mpeg1:EGFP) control larvae. Triangles indicate positive signals. (D, H) Statistical result for A–C’ and E–G’. Data shown are the means ± standard error of the mean (SEM) of at least 15 larvae. ns: not statistically significant, **p<0.01. (I) Classification of zebrafish monocytes/macrophages is based on their morphology. (J) Quantification of mpeg1.1 ⁺ cells at each differentiation stage. Sorted macrophage lineage cells from 10-month-old zbtb14^-/-//Tg(mpeg1.1:eGFP) and Tg(mpeg1.1:eGFP) larvae were subjected to May-Grünwald-Giemsa staining and separated into immature, intermediate, and mature groups according to their morphology. Scale bar: 10 µm. Error bars represent mean ± SEM of three independent experiments. *p<0.05 (Student’s t-test).

Acknowledgments

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