Fig. 2

vTel^y321 neurons are both cholinergic and GABAergic. A Coronal sections of the adult ventral forebrain in the rostral telencephalon showing expression of the Et y321 transgene-dependent GFP, mRNA expression of vachtb and GABA immunolabeling. A’ Insets of boxed areas in A. Arrowheads point to selected vTel^y321 neurons that express vachtb and GABA (Scale bars: A = 100 μm; A’ = 50 μm). B Coronal sections of ventral forebrain depicting some nuclei in the forebrain that include vTel^y321 neurons and which provide anatomical positions used for rostro-caudal plane identification prior to quantification of vTel^y321 CGNs (RT, rostral telencephalon; MT, mid-telencephalon; and CT, caudal telencephalon; Vv, Ventral nucleus of the ventral telencephalon; Vd, dorsal nucleus of the ventral telencephalon; Vs, supracommisural nucleus; PPa, Parvocellular pre-optic nucleus; ac, anterior commissure) (scale bar: B = 100 μm). C Boxplots showing percentage of adult vTel^y321 neurons that are cholinergic and GABAergic at selected anatomical forebrain landmarks. N = 4