ZFIN Image: Field et al., 2022, FIGURE 4

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Figure Caption

FIGURE 4

tmem184a-∆CT mRNA does not rescue knockdown phenotypes. Embryos were injected with (A,A′) SC MO (n = 20) (B,B′) SB MO followed by 25 ng/μL full-length Tmem184a mRNA (n = 60) (C,C′) SB MO (n = 40) (D,D′) SB MO followed by 25 ng/μL tmem184a-ΔCT (n = 60) or (E,E′) SB MO followed by 100 ng/µL tmem184a-ΔCT (n = 60) and evaluated at 48 hpf as described in Methods. (F) The graph illustrates the intact ISVs in SC, rescue (SB MO plus full-length Tmem184a mRNA as in Figure 2) embryos, SB MO plus tmem184a-ΔCT at either concentration. p < 0.001. (G) The distance between ISVs was measured for each treatment using Image J. P < 0.001. (H) Embryo survival for all treatments illustrated relative to 24 h survival of SC embryos.

Acknowledgments

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