FIG 5

FIG 5

Transcriptomic analysis of zebrafish larvae. (A–C) Volcano plots of DEGs. Log₂ (Fold change) ≥1 was set as the threshold. (D–F) Pathway classification based on KEGG enrichment analysis of DEGs. We enriched the differential genes according to Log₂ (Fold change) ≥1, upregulated genes P < 0.1, and downregulated genes P < 0.05 for KEGG functional enrichment. We selected the top 20 pathways for presentation. Red: Glycan biosynthesis and metabolism. Blue: lipid metabolism. Green: immune system. Yellow: carbohydrate metabolism. Gray: amino acid metabolism. ETEC, WT+ETEC and ΔluxS+ETEC, zebrafish larvae in germ-free were exposed to none or to wild-type or ΔluxS LGG at a concentration of 10⁸ CFU/mL for 24 h on 5 dpf, respectively, before being exposed to ETEC at a concentration of 10⁸ CFU/mL for 24 h on 6 dpf.