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Fig. 7

ID
ZDB-IMAGE-220822-22
Source
Figures for Agarwala et al., 2022
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Figure Caption

Fig. 7

Dopamine beta-hydroxylase positive ganglion-like cells are present within the larval kidney marrow niche.

(A) 3D rendering generated using light sheet movies of Runx:mCherry;dbh:GFP double transgenic larva shows mCherry+ clusters (dotted ovals) in close proximity to GFP+ cells. DA, dorsal aorta. (B) Oblique slice through the 3D volume shows GFP+ extensions from the dbh:GFP+ cells into the mCherry+ hematopoietic stem and progenitor cell (HSPC) clusters. (B’) Detail of the boxed region in B shows contact formation between the GFP+ extensions and mCherry+ HSPCs (white arrowheads) in all three planes. (C) Quantification showing significantly reduced number of Runx:mCherry+ HSPCs in 6-hydroxydopamine (6-OHDA)-treated transgenic larvae compared to DMSO controls. Unpaired t test with Welch’s correction. DMSO vs. 250 μM 6-OHDA, p=0.0003; DMSO vs. 300 μM 6-OHDA, p=0.0002. Sample size (n), DMSO, n=13; 250 μM 6-OHDA, n=18; 300 μM 6-OHDA, n=15. Number of biological replicates = 3. (D) The ultrastructure of a dbh:GFP+ cell (labeled green) in proximity of a cell with HSPC-like morphology (labeled magenta) (white arrowheads). Surrounding tissues are labeled, and the dbh:GFP+ cell HSPC pair is posterior to the glomerulus, dorsal to pneumatic duct, pronephric tubule and intestine, and ventral to the notochord. (D’) Higher magnification shows the dbh:GFP+ cell-HSPC pair is only three-cell diameters from the vessel lumen (white area). (D’’) Full resolution detail of the dbh:GFP+ cell-HSPC pair showing contact formation. Abbreviations: D, dorsal; V, ventral; A, anterior; P, posterior.

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