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Fig. 2
A,B. Transmission efficiency of the second stage as a function of the acoustic frequency driving the AOD. The bandwidths addressed in the ST and MT modes are colored in blue and orange, respectively. A, Low chirp case: α = 0.2 MHz/μs. B, High chirp case: α = 0.5 MHz/μs. The number of triggers is indicated for the MT modality. C, Top: Schematic of the RF signals used to drive each AOD in the ST (blue) and MT (orange) modes. After the chirp α is defined, a frequency ramp is generated once (single trigger) or repeated multiple times (multitrigger) within the dwell time window. Bottom: The simulated integrated signal collected by the PMT. D, Normalized fluorescence signal collected by the PMT as a function of the applied chirp, addressing light in separate planes with ST (blue) and MT (orange) configurations; 2D raster scans are obtained by illuminating 10 × 10 points with a dwell time of 20 μs. The integration time of the PMT was set consistently to 20 μs. AOD, Acousto-optic deflector; MT, multitrigger; ST, single trigger
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Full text @ J. Biophotonics