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FIGURE 4 The CRM in the Robo3 locus is not conserved in teleosts. (a) ChIP-seq and ATAC-seq peak call results are displayed in the UCSC genome browser with Multiz Alignments track. The Robo3 locus in the mouse genome (mm10) is displayed. The region harboring multiple TF binding sites (Robo3-CRM) is highlighted in yellow. Multiz Alignment tracks of tetrapods and teleosts are highlighted by different colors. (b–i) Robo3-CRM::GFP was electroporated into chick neural tubes. (b, c) The expression of GFP and ROBO3 was examined at HH20 (b) and HH25 (c) after electroporation. (d–i) The expression of the GFP and TFs indicated was examined at HH25 after electroporation. The edges of the neural tube are demarcated by the broken lines. The embryo numbers examined in the electroporation experiments are provided in Table S5. Scale bar: 100 μm in c for b and c, in i for d–i