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Fig. 4
Fig. 4. Cytometric quantification of HAB (5 µM) in live L. amazonensis promastigotes (20,000 cells/µL) in presence of 7-AAD (20 µM) and presence / absence of Amp B (2 µM). A, E, I and M: morphological biparametric plots (FS, cell size; SS, cell granularity); B, F, J and N: gated plots (FL4: 7-AAD, FL10: HAB); C, G, K and O: 7-AAD cytometric histograms; D, H, L and P: HAB cytometric histograms. Plot data are representative of three independent experiments. Means and standard deviations of cell count and fluorescence intensity data (n = 3) are indicated in Table 1.
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