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Fig. 5
DSF reduced LPS-induced apoptosis in zebrafish embryos. (A) The prevalence of apoptosis in zebrafish embryos was detected with fluorescent dye AO staining; red arrows indicate the apoptotic cells. (B) The fluorescence intensity was quantified for individual zebrafish using ImageJ analysis. (C) The relative mRNA expression levels of apoptosis markers (baxa, bcl2, mdm2, and tp53) were measured with qRT-PCR. Data are represented as mean ± S.D. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; ## p < 0.01, and ### p < 0.001 vs. LPS group. Scale bar, 200 μm.
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