ZFIN Image: Sarohi et al., 2022, Fig. 8

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Figure Caption

Fig. 8

Quantitation of microheterogeneity of K¹⁰¹⁷ site in COL1A1a present in zebrafish heart ECM during regeneration. (A) Chromatogram plots represent the elution of unmodified (K), hydroxylysine (HyK), and glucosylgalactosyl-hydroxylysine (GG-HyK) modified K¹⁰¹⁷ site containing peptide ¹⁰¹¹DGAAGPKGDRGETGPSGTPGAPGPPGAAGPIGPAGK¹⁰⁴⁶ (B) Graphical representation of Skyline-based MS¹ quantitation of micro-heterogenic distribution of unmodified K¹⁰¹⁷ (yellow), HyK¹⁰¹⁷ (green) and GG-HyK277 (blue) species in COL1A1a from the ECM digest of control, 7 DPA, 14 DPA, and 30 DPA regenerating zebrafish heart. The different colors in the bar represent the occupancy of different forms at the K¹⁰¹⁷ site in COL1A1a with mean ± SEM. An increase in glucosylgalactosyl-hydroxylysine levels during regeneration is significant (ANOVA, p < 0.05) (See Table 3)

Acknowledgments

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