ZFIN Image: Lu et al., 2022, Fig. 1

Image

Figure Caption

Fig. 1

Sex-dependent fish susceptibility to mortality by SVCV infection.

(A-B) Survival (Kaplan-Meier Curve) of male and female zebrafish (n = 30 per group) at various days after i.p. injected with SVCV (5 × 10⁸ TCID₅₀/ml, 5 μl or 10 μl/individual). (C) Wild-type Male and female gibel carp (n = 35 per group) were i.p. injected with CaHV (5 × 10⁷ TCID₅₀/ml, 30 μl/individual) and the survival rate was calculated. (D-F) Microscopy of H&E-stained spleen (D), heart (E), and gonad (F) sections from male and female zebrafish treated with SVCV (5 × 10⁸ TCID₅₀/ml, 5 μl/individual) for 72 h. (G) qPCR analysis of ifnφ1 mRNA in the spleen and kidney of male and female zebrafish (n = 8 per group) given i.p. injection of SVCV (5 × 10⁸ TCID₅₀/ml, 5 μl/individual) for 48 h. Each dot point represents one independent biological replicate. *p < 0.05. (H) qPCR analysis of Cgifn-A mRNA in the spleen and kidney of male and female gibel carp (n = 8 per group) given i.p. injection of CaHV (5 × 10⁷ TCID₅₀/ml, 30 μl/individual) for 48 h. Each dot point represents one independent biological replicate. *p < 0.05. (I) qPCR analysis of svcv-n mRNA in the spleen and kidney of zebrafish (n = 8 per group) given i.p. injection of SVCV (5 × 10⁸ TCID₅₀/ml, 5 μl/individual) for 48 h. *p < 0.05.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.