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Fig. 8 Efficacy of the PACT compound [2](PF6)2 in the Tg(Fli:GFP/casper) zebrafish orthotopic model of conjunctival melanoma (CRMM1 and CRMM2 cell lines) by retro-orbital administration (RO). (a) Scheme showing the injection site of retro-orbital administration. (b) Time flow of [2](PF6)2 treatment with RO administration. Around 200 CRMM1 or CRMM2 cells were injected into the RO site of zebrafish embryos at 2 dpf. [2](PF6)2 was injected into RO site at 3, 4, 5, 6 dpf, and after 1 h drug-light interval, the embryos were irradiated with green light (520 nm, 21 mW cm−2, 90 min, 114 J cm−2). (c) The images of CRMM1 or CRMM2 tumour burden (in red) at CHT site at 6 dpf. Green represents vessels in zebrafish embryos. (d and g) The relative red fluorescence (excitation: 554 nm, emission: 581 nm) intensity of CRMM1 or CRMM2 tumour burden at 6 dpf. (e and h) The relative tumour area (pixel2) of CRMM1 or CRMM2 tumour burden at 6 dpf. Results are presented as means ± SD from three independent experiments. ****P < 0.0001.