ZFIN Image: Bruton et al., 2022, Fig. 3

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Figure Caption

Fig. 3

Figure 3. Macrophages not required for the recovery of cardiac structure or function

(A) Representative GFP surface renders of LSFM z stacks of injured ventricles in Tg(myl7:GFP;csfr1a:NfsB-mCherry) larvae, macrophage ablation groups as indicated in the figure. Abluminal myocardial surface is shown at 6 hpi (left), and abluminal and luminal surfaces are shown at 48 hpi following regeneration (middle and right). White arrowheads, laser lesion.

(B) Quantification of mean lesion size in injured Tg(myl7:GFP;csfr1a:NfsB-mCherry) larvae per macrophage ablation group, n = 11–22.

(C) Quantification of ventricular ejection fraction in uninjured and injured Tg(myl7:GFP;csfr1a:NfsB-mCherry) larvae per macrophage ablation group, n = 10–12.

(D) Representative GFP surface renders of LSFM-acquired z stacks of injured ventricles from irf8^+/+ and irf8^−/− Tg(myl7:GFP) larvae. Abluminal myocardial surface is shown at 6 hpi (left), and abluminal and luminal surfaces are shown at 48 hpi following regeneration (middle and right). White arrowheads, laser lesion.

(E) Quantification of mean lesion size in injured irf8^+/+ and irf8^−/− Tg(myl7:GFP) larvae, n = 15.

(F) Quantification of ventricular ejection fraction in uninjured and injured irf8^+/+ and irf8^−/− Tg(myl7:GFP) larvae n = 15–20.

(G) Time-lapse time points of a GFP-surface-rendered, injured Tg(myl7:GFP) ventricle from 6 hpi. White box, zoom panel; white arrowheads, myocardial buds; cyan arrowhead, myocardial bridge. ^∗ p ≤ 0.05, ^∗∗∗ p ≤ 0.001. Two-way ANOVA followed by Holm-Sidak’s post-hoc tests. Scale bars, 50 μm. Data are represented as mean ± SEM.

Acknowledgments

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Reprinted from Developmental Cell, 57(12), Bruton, F.A., Kaveh, A., Ross-Stewart, K.M., Matrone, G., Oremek, M.E.M., Solomonidis, E.G., Tucker, C.S., Mullins, J.J., Lucas, C.D., Brittan, M., Taylor, J.M., Rossi, A.G., Denvir, M.A., Macrophages trigger cardiomyocyte proliferation by increasing epicardial vegfaa expression during larval zebrafish heart regeneration, 1512-1528.e5, Copyright (2022) with permission from Elsevier. Full text @ Dev. Cell