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Fig. 1
The pancreatic cancer cells show differential engraftment profile in zebrafish. (A) The growth curve of in-vitro cultured PANC1, BxPC3, and AsPC1 cells. (B) Representative confocal images of PANC1, BxPC1, and AsPC1 xenografts at 1 dpi and 4 dpi. (C) Engraftment quantification for PANC1, BxPC1, and AsPC1 cells at 4 dpi. Results are shown as means ± SEM from 50 different individuals. (D) Relative tumor growth (4 dpi vs. 1 dpi) for PANC1, BxPC3, and AsPC1 xenografts. Results are from nine different individuals (*** p < 0.001, **** p < 0.0001, t test). (E) PANC1 and AsPC1 showed metastasis in the intestine (white arrowheads) and in the caudal hematopoietic tissue (white arrows). (F) Representative confocal images of PANC1, BxPC3, and AsPC1 xenografts at 1 dpi or at 4 dpi after DMSO (control), irinotecan (50 μM) or 5-Fu (2 mM) treatment. (G) Relative tumor growth (4 dpi vs. 1 dpi) for PANC1, BxPC3, and AsPC1 xenografts after treatment with DMSO, irinotecan or 5-Fu. Results are from nine different individuals (* p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001, t test). dpi, days post injection. Scale bars in (B,F), 50 µm; in (E), 75 µm.