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Fig. 2
SH suppressed NF-κB translocation by reducing the phosphorylation of IκB-α in THP-1 macrophages. THP-1 macrophages were co-treated with SH and LPS (1 μg/ml) for 12 h. (A) Relative phosphorylation of IκB-α and total protein levels as determined by western blot. (B) Nuclear translocation of NF-κB (p65) as determined by western blot, and (C) visualized by immunofluorescence microscopy. Results are presented as the means ± SDs of three different experiments. ##p < .01 versus non-treated control macrophages, and *p < .05, **p < .01 versus LPS-treated control macrophages.
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Full text @ FASEB J.