Figure 1

Figure 1

nlrc3-like deficiency promotes host control of the mycobacterial proliferation during early infection. (A) Zebrafish embryo infections and bacterial fluorescence intensity. The red arrow indicates the duct of Cuvier, the site where bacteria were injected. (B) Representative images for M. marinum:Wasabi infected embryos at 3 dpi. (C) Statistics of relative green fluorescence intensity at the indicated time points taken from two independent biological replicates. (D) Process for calculating granuloma-like structure in zebrafish embryos: i original image; ii. the image after fluorescence-pixelation processing – the area of ​​the red pixels represents the relative size of each granuloma; iii. Calculation of the area of ​​the red pixels. (E) Graph showing the number of large, small and all granuloma-like structures in nlrc3-like -/- and control embryos. (B, D) Scale bar = 200 μm *p ≤ 0.05; **p ≤ 0.01; ns, not statistically significant (p>0.05)..