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Fig. 6 A Sequence alignment of c-termini of eIF4E2 from major species, including mammals, reptiles, amphibians, fish, and birds. B WB detects the protein expression of eIF4E2-withGβ isoforms in different non-mammals. C Human eIF4E2 isoform A prevents disorder of heart looping of zebrafish. Vectors expressing eIF4E2 isoform A were mock-injected or injected into embryos (n = 5) at the 1-cell stage. 24 h later, embryos were exposure to hypoxic (1% O2) or normoxia for 24 h, and representative Tg (cmlc2: eGFP) heart were showed at 48 h. D, E Expression of eIF4E2 isoform A increases the mRNA expression of TOPBP1 or TRX1 in zebrafish embryo. Total RNA was extracted and reverse transcriptase polymerase chain reaction (RT-PCR) was performed (D). Real-time quantification reverse transcriptase polymerase chain reaction (QRT-PCR) was performed (E). F S/T-P sites of zebrafish p53 were indicated. G Zebrafish p53-4A mutant suppresses the expression of TOPBP1 or TRX1. Vectors expressing Zebrafish p53-4A, were mock-transfected or transfected into p53-null HCT116 cells for 48 h, followed by WB. H TANNylated e2-I or e2-S was intravenously injected to mice twice a week for 6 weeks, followed by intermittent hypoxia (IH) exposure (12% O2 for 8 h after injection). Cardiac fibrosis was evaluated by Masson’s trichrome staining and representative macroscopic photographs of heart tissue was showed (n = 4) (left panel). Quantified data of the fibrosis area. Statistical significance was determined by one-way analysis of variance (n = 4) (right panel). I Mice were intravenously injected with TANNylated e2-I or e2-S, and housed at physiological hypoxia (12% O2) conditions for 48 h. Then, heart tissues were analyzed by WB.