ZFIN Image: Wu et al., 2022, FIGURE 4

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Figure Caption

FIGURE 4

The effect of promoter hypermethylation on the expression of miR-29b-3p. (A and B) The methylation levels of the pGL3-miR-29b-1 promoter and pGL3-miR-29b-2 promoter before M. SssI treatment. For each plasmid, the methylation status of CpG units is shown for ten clones. Black and white circles indicate the methylated and unmethylated CpG units, respectively. (C and D) The methylation levels of the pGL3-miR-29b-1 promoter and pGL3-miR-29b-2 promoter after M. SssI treatment. (E) The effect of methylation on the transcriptional activity of the −873 bp to +158 bp region of the miR-29b-1 gene. (F) The effect of methylation on the transcriptional activity of the −1407 bp to −1173 bp region of the miR-29b-2 gene. (^ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001).

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