Figure 4

Figure 4 Figure 4. Effects of 5-Aza and miR-139-5p on ROCK2 expression. (a) Effects of 5-Aza on ROCK2 protein level in JHH-6. Left, representative Western blot (GAPDH was introduced for normalization); right, data normalized to GAPDH levels and to the average of non-treated cells (NTC) are reported in % as mean ± SEM, n = 6; NTC vs. 0.8 μM, * p = 0.0398; NTC vs. 6 μM, ^# p = 0.0177. (b) Effects of 5-Aza on ROCK2 protein level in HuH-7. Left, representative Western blot; right, data normalized to GAPDH levels and to the average of NTC are reported in % as mean ± SEM, n = 4; NTC vs. 0.8 μM, * p = 0.0135; NTC vs. 6 μM, ^# p = 0.0081; NTC vs. 30 μM, ^§ p = 0.038. (c) Effects of 5-Aza on ROCK2 mRNA level in JHH-6. Data normalized to 28S levels and to the average of NTC are reported in % as mean ± SEM, n = 6; NTC vs. 0.8 μM, * p = 0.0025; NTC vs. 6 μM, ^# p = 0.0001. (d) Effects of 5-Aza on ROCK2 mRNA level in HuH-7. Data normalized to 28S levels and to the average of NTC are reported in % as median with interquartile range, n = 6; NTC vs. 6 μM, * p = 0.0022; NTC vs. 30 μM, ^# p = 0.002. (e) Effects of miR-139-5p on ROCK2 protein level in JHH-6. Left, representative Western blot; right, data normalized to GAPDH levels and to the average of scr (scrambled miR treated cells) are reported in % as mean ± SEM, n = 5; scr vs. miR-139-5p, * p = 0.0071. (f) Effects of miR-139-5p on ROCK2 protein level in HuH-7. Left, representative Western blot; right, data normalized to GAPDH levels and to the average of scr are reported in % as mean ± SEM, n = 5; scr vs. miR-139-5p, * p = 0.0268. (g) Effects of miR-139-5p on ROCK2 mRNA level in JHH-6 and HuH-7. Data normalized to 28S levels and to the average of scr are reported in % as mean ± SEM, n = 3; scr vs. miR-139-5p, * p = 0.04.