Figure 2

Figure 2 Figure 2. Effects of 5-Aza on cell cytoskeleton. (a,b) Immunostaining of β-actin and β-tubulin in JHH-6 and HuH-7 cells, respectively. Images were acquired with a Leica DM 2000 microscope. Red = β-actin; green = β-tubulin; blue = DAPI. Magnification 40X (bar = 100 μm). (c) Ratio of polymerized/cytosolic α-tubulin in 5-Aza-treated vs. non-treated cells (NTC); data are expressed as mean ± SEM; JHH-6 NTC vs. JHH-6/5-Aza, ^§ p= 0.04; HuH-7 NTC vs. HuH-7/5-Aza, ^$ p= 0.045, n = 3. (d,e) Quantification of the number of altered nuclei shape in 5-Aza-treated vs. non-treated cells. Data, expressed as mean ± SEM, are reported as percentage normalized to the average of non-treated cells (NTC). Abnormal nuclei in JHH6/NTC vs. JHH6/0,8-6 μM, * p < 0.0001 n = 60; HuH7/NTC vs. HuH7/6-30 μM, ^# p < 0.001 n = 60.