Figure 1.

Figure 1.

egr1 is expressed in the forebrain. A, ISH reveals egr1 expression (blue) in the basal forebrain (BF), optic tectum (OT), hypothalamus (Hyp), and epithelial cells of the pharyngeal pouch (PP) at 6 dpf. myogenin d was used as a control probe (red). Anterior to the left. Scale bar: 100 μm. B, Magnification of the BF (white box in A) showing expression surrounding the anterior commissure (AC). C, Exon organization of the egr1 mRNA, location of the zinc finger domains (zf), and location of the sa64 mutation (red bar). D, egr1^sa64 mutants (−/−) develop a normal body plan with functional swim bladder, but with size deficits (bottom). E, Close to 80% of mutant larvae are stunted (dark gray). F, More than 50% of stunted larvae are also wt (+/+, white) and het animals (+/−, light gray). N = 8 clutches, n = 248 animals.