IMAGE

Fig. 7

ID
ZDB-IMAGE-220411-15
Antibodies
Source
Figures for Abrial et al., 2022
Image
Figure Caption

Fig. 7

TGFβ signaling protects larval zebrafish from OFT aneurysm and ventricular dilation. (A) Experimental timeline for small-molecule-mediated inhibition of TGFβ signaling in wild-type animals. (B,C) Brightfield images of 5 dpf wild-type larvae treated with DMSO (B) or LY364947 (C). Arrowhead and * in C highlight a jaw protrusion and mild pericardial edema, respectively, observed in LY364947-treated animals. (D-F) Confocal images of hearts in 5 dpf wild-type animals treated with DMSO (D) or LY364947 (E,F) and double immunostained for striated muscle (MF20, red) and Eln2-positive OFT smooth muscle (green). E and F show representative hearts with moderate and severe phenotypes, respectively. Ventricular size was measured by quantifying the area within the perimeter of the chamber (areas encircled by dashed lines in D-F). (G) Dot plot showing the ventricular areas of 5 dpf wild-type larvae treated with DMSO (n=8) or LY364947 (n=11). (H-J) Single optical sections taken from the images shown in D-F through the OFTs of 5 dpf wild-type larvae treated with DMSO (H) or LY364947 (I,J). White lines show the maximal OFT diameters between the Eln2-positive walls of smooth muscle. (K) Dot plot showing the OFT diameters in DMSO-(n=5) and LY364947-(n=8) treated animals. (L-N) Confocal images of OFTs in 5 dpf wild-type animals treated with DMSO (L) or LY364947 (M,N) double immunostained for striated muscle (MF20, red) and phosphorylated Smad3 (pSmad3; green). (O) Dot plot showing the relative mean pSmad3 fluorescence intensities in the OFTs of DMSO-(n=9) or LY364947-(n=9) treated larvae. For all dot plots, statistical significance was determined with an unpaired t-test. Error bars show one standard deviation. **P<0.01. Scale bars: 20 µm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.