Figure 1.

Figure 1.

A schematic showing the experimental process and example data from in vivo electrophysiological recording in 4-d post fertilisation elavl3:GCaMP6s zebrafish larvae. A, The experimental process used. B, Left, A paralyzed, mounted zebrafish larva with glass electrode inserted into midbrain. The Red circle indicates the placement of the tip of the electrode, while the blue dashed line indicates landmarks used to consistently place the electrode. Right, local field potential recording (LFP) from midbrain of zebrafish larva at baseline and after administration of 30 μm picrotoxin.