Fig. 4.

Fig. 4.

Short-term inhibition of VEGF reduces vascular topology parameters. (A) MIPs of averaged data of control and AV951-treated samples following segmentation and registration showed no visual phenotype [PMBC pattern (blue arrowhead), head size (PMBC′ to PMBC″ distance; cyan dotted line), BA (green arrowhead), CtAs (yellow arrowhead) and PHBC (magenta arrowhead)]. (B) No statistically significant difference was found when comparing registered controls with VEGF inhibitor-treated samples (P>0.9999; control=18, AV951=23; Kruskal–Wallis test; data are mean±s.d.). (C) Vascular volume was statistically significantly decreased in AV951-treated samples (P=0.0014; control=22, AV951=23; two-tailed Mann–Whitney U-test; data are mean±s.d.). (D) Vascular surface was statistically significantly decreased in AV951-treated samples (P=0.0010; two-tailed Mann–Whitney U-test; data are mean±s.d.). (E) Vascular density was not statistically significantly changed in AV951-treated samples (P=0.1048; unpaired two-tailed Student's t-test; data are mean±s.d.). (F) Branching points were statistically significantly decreased in AV951-treated samples (P=0.0016; two-tailed Mann–Whitney U-test; data are mean±s.d.). (G) Vascular network length was statistically significantly changed in AV951-treated samples (P=0.0004; two-tailed Mann–Whitney U-test; data are mean±s.d.). (H) Average vessel radius was statistically significantly reduced in AV951-treated samples (P=0.0371; unpaired two-tailed Student's t-test; data are mean±s.d.). (I) Vascular complexity was not statistically significantly changed in AV951-treated samples (P=0.4949; two-tailed Mann–Whitney U-test; data are mean±s.d.).