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Fig. 7 (A-G). Effect of lactalbumin hydrolysate administration on nutrient-sensing related genes in larval trunk (8 dpf). Values are mean ± SE (n = 8). Differences in tube-feeding test with respect to the values on the 0% hydrolysate group (indicated by †) within each zebrafish line (lepr−/−or wt) were assessed by one-way ANOVA. Differences between the lines at each sampling time (indicated by *) were assessed by two-way ANOVA. Two-way ANOVA shows no significant effect of concentration [F (3, 53) = 2.292; p > 0.05] but significant effect of genotype [F (1, 53) = 14.30; p < 0.0001] at 1 h, and no significant effect of concentration [F (3, 54) = 0.9335; p > 0.05] but significant effect of genotype [F (1, 54) = 11.70; p < 0.01] at 3 h for casr; significant effects of concentration [F (3, 56) = 12. 14; p < 0.0001] and genotype [F (1, 56) = 7.005; p < 0.05] at 1 h and significant effects of concentration [F (3, 56) = 13.92; p < 0.0001] and genotype [F (1, 56) = 35.26; p < 0.0001] at 3 h for pept1a; significant effect of concentration [F (3, 56) = 10.91; p < 0.0001], but not genotype [F (1, 56) = 3.658; p > 0.05] at 1 h and significant effects of concentration [F (3, 56) = 20.15; p < 0.0001] and genotype [F (1, 56) = 42.18; p < 0.0001] at 3 h for pept1b; no significant effect of concentration [F (3, 56) = 0.5851; p > 0.05], but significant effect of genotype [F (1, 56) = 26.76; p < 0.0001] at 1 h and no significant effects of concentration [F (3, 55) = 2.728; p > 0.05] and genotype [F (1, 55) = 2.754; p > 0.05] at 3 h for t1r1; no significant effects of concentration [F (3, 52) = 2.435; p > 0.05] and genotype [F (1, 52) = 0.5828; p > 0.05] at 1 h and significant effects of concentration [F (3, 49) = 3.988; p < 0.05] and genotype [F (1, 49) = 8.861; p < 0.01] at 3 h for t1r2-1; no significant effect of concentration [F (3, 56) = 0.2170; p > 0.05] but significant effect of genotype [F (1, 56) = 64.26; p < 0.0001] at 1 h and no significant effect of concentration [F (3, 54) = 0.1986; p > 0.05] but significant effect of genotype [F (1, 54) = 43.93; p < 0.0001] at 3 h for t1r2-2; no significant effects of concentration [F (3, 53) = 0.7686; p > 0.05] and genotype [F (1, 53) = 1.129; p > 0.05] at 1 h and no significant effects of concentration [F (3, 55) = 1.748; p > 0.05] and genotype [F (1, 55) = 1.063; p > 0.05] at 3 h for t1r3. A Dunnett’s multiple comparison test for one-way ANOVA and a post-hoc Turkey’s multiple comparison were used to assess for specific pairwise differences (*, † = p < 0.05; **, †† = p < 0.01 and ***, ††† = p < 0.001).