ZFIN Image: Swain et al., 2022, Figure 3

Image

Figure Caption

Figure 3

Complementation of ΔasdA mutant strains with E. piscicida AsdA⁺ Plasmids. (A)E. piscicida AsdA⁺ Plasmid maps (pG8R8011-pG8R8018) showing origin of replication, orientation of asdA gene, presence or absence of the promoter for the asdA gene and with either ATG or GTG start codons, P_trc promoter and multiple cloning sites. (B) Characteristic of E. piscicida AsdA⁺ plasmids or various strategies to construct AsdA⁺ plasmids via modification of the plasmid copy number (pUC ori and pBR ori), promoter, SD sequence and start codon. (C) Growth of ΔasdA (χ16000) mutant strains complemented with different E. piscicida AsdA⁺ plasmids (pG8R8011 - pG8R8018).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol