ZFIN Image: Janiak et al., 2022, Fig. 8

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Figure Caption

Fig. 8

Mesoscale and random-access imaging in mouse brain slice.

a, b Schematic of brain (a) and transverse section (b) of a Thy1:GCaMP6f mouse. c, d 1024 × 1024 px nTC₂ example scan of slice through cortex and hippocampus at maximal FOV (c) and nTC₂ zoom in (d) as indicated. Red arrows indicate rapid transitions between scan regions, with the inset showing scan-profiles. The slice was bathed in an epileptogenic (high K⁺, zero Mg²⁺) solution to elicit seizures. e–g Mean of 256 × 256 px scan (3.91 Hz) of (d) with regions of interest (ROIs) indicated (e), activity-correlation projection (Methods) indicating regions within the scan showing regions of activity computed as mean correlation of each pixel’s activity over time to all its neighbours (for details, see ref. ^⁷⁰) (f) and z-normalised fluorescence traces (g). h–l, 2 times 128 × 256 px (3.91 Hz) random access scan of two regions as indicated in (d) allows quasi-simultaneous imaging of the cortex (h) and hippocampus (i) at increased spatial resolution, with activity-correlation (j, k, cf. Fig. 3c) and fluorescence traces (l) extracted as in (j, k).

Acknowledgments

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