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Fig. 4 Chemoptogenetic cardiomyocyte ablation causes arrhythmia. A, Representative electrocardiograms (ECG) of adult zebrafish before and after amputation or ablation. One day prior amputation or ablation (left panels), hearts showed normal heart beat rhythm without arrhythmic events. Seven days post amputation or MG-2I injection and IR light treatment, several zebrafish showed arrhythmic events (central panels) Red double heads arrows indicate longer or shorter R-R intervals. Importantly, injection and irradiation of control MG-ester did not cause arrhythmia (central panels). Forty-five days post injury (right panels), ECG were comparable to uninjured hearts, suggesting that all the hearts have recovered. B, Graph quantifying the ECG. At least n = 7 for each group. *P < .05; ****P < .0001; one-way ANOVA. Each ECG trace revealed at least 250 heartbeats