Fig. 4

Fig. 4 CRHR mediates the effects of CRH on zebrafish ovary. (A and B) Follicular cells were treated with or without CRHR inhibitor (α-helical 9–41) (10 μM) for 1 h, followed by 100 nM CRHα or 100 nM CRHβ treatment for 5 min and 10 min, respectively. Representative blots and quantified graphs (n = 3) are shown. (C and D) Follicular cells were treated with or without CRHα or CRHβ (100 nM) in the presence or absence of α-helical 9–41 for 24 h. The mRNA expression of cyp19a1a, hsd17b1, and hsd17b3 were detected using qPCR (n = 4). The content of E2 in the medium was measured by ELISA (n = 4). Different lowercase above columns indicates statistical significance (P < 0.05).