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FIGURE 7 Limonin protected zebrafish larvae against oxidative stress. (A) Fluorescence micrographs of DHE. Figures are magnified as 50×. (B) Fluorescence micrographs of NA-8. Figures are magnified as ×50 (C) ROS quantification according to the mean intensity of red fluorescence. (D) GSH quantification according to the mean intensity of blue fluorescence. (E) Real-time PCR analysis of gpx1a mRNA levels in zebrafish larvae. The mRNA levels were normalized to β-actin mRNA levels and presented as fold change compared with the control group. Data are shown as the mean ± SD (n = 4–6 per group). #p < 0.05, ##p < 0.01, ###p < 0.001 vs control group; *p < 0.05, **p < 0.01, ***p < 0.001 vs TAA group.