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FIGURE 6 Limonin reduced liver inflammatory damage caused by TAA. (A) Limonin reduced the infiltration of macrophages in the liver of zebrafish during TAA exposure. Figures are magnified as ×25 and 50× (n = 6 per group). (B) Relative expression of mpeg1 in zebrafish liver. (C,F) Immunohistochemical staining of TNF-α and F4/80 in paraffin sections of zebrafish larvae (n = 4 per group). (D,G) Quantify the fluorescence intensity of TNF-α and F4/80 proteins. (E,H) Real-time PCR analysis of IL-1β and IL-6 mRNA levels in zebrafish larvae. The mRNA levels were normalized to β-actin mRNA levels and presented as fold change compared with the control group (n = 3 per group). Data are shown as the mean ± SD. #p < 0.05, ##p < 0.01, ###p < 0.001 vs control group; *p < 0.05, **p < 0.01, ***p < 0.001 vs TAA group.