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FIGURE 4 Limonin reduced lipid accumulation caused by TAA in zebrafish liver. (A) Oil red O staining of frozen sections of zebrafish larvae. (B) Frozen liver sections of zebrafish larvae with liver-specific eGFP expression were stained with Nile Red. Figures are magnified as ×200. (C) Quantitative analysis of the area of lipid droplet in liver based on Oil Red O staining. (D) Quantitative analysis of the liver mean fluorescence intensity of Nile Red. (E) Real-time PCR analysis of FASN mRNA levels in zebrafish larvae. The mRNA levels were normalized to β-actin mRNA levels and presented as fold change compared with the control group. Data are shown as the mean ± SD (n = 6–8 per group). #p < 0.05, ##p < 0.01, ###p < 0.001 vs control group; *p < 0.05, **p < 0.01, ***p < 0.001 vs TAA group.