Immunofluorescent staining showing the expression of (A) Frizzled 9 (red) within the epithelial and neuromuscular compartments; (B) PAN neuronal marker (green) within the neuromuscular layer; and (C) both markers (Frizzled 9 and PAN neuronal marker) in colocalization (yellow); and (D) Wnt3a within the epithelium and neuromuscular layer. Nuclear counterstaining was performed using DAPI (blue). (E) Whole-mount immunofluorescent staining of zebrafish ENS network by detection of PAN neuronal marker (green) Bar: 50 µm. Optical microscopy of (F) primary intestinal epithelial cells, characterized by the expression of Pan-cytokeratin (left corner, green); ENS cultures at (G) 24 h and (H) 7 days from isolation. Bar: 100 µm (200 µm in left corner). (I) Flow cytometry analysis of Sox2 and Sox10 in ENS cells at the time of isolation (T0) and after culturing for 7 days (T7). Data are expressed as percentage (%) of positive cells ± standard deviation (SD). Created with BioRender.com, accessed on 31 October 2021.
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Pharmaceuticals (Basel)