Figure 3

Figure 3

Reconstructing bipolar cell responses from cones

(A–E) Summary of the reconstruction strategy for example cluster C₂₂ (for details, see STAR Methods). Each BC-cluster reconstruction is based on the linear combination of the spectral tuning functions of the four cone types (A; from Yoshimatsu et al.²⁷) with four stereotyped temporal components associated with individual light flashes (B), yielding 4 × 4 = 16 weights (C). Weights are shown in blocks of temporal component weights (from left: light transient; light sustained; dark transient; and dark sustained) associated with each cone (indicated by the corresponding colors). Bars above zero indicate sign-inverted (“On”) weights, while bars below zero indicate sign-conserved (“Off”) weights. The corresponding full expansion of this reconstruction is shown in (D). Individual combination of each cone’s tuning function (A) with each temporal component (B), scaled by their corresponding weight (C), yields sixteen “sub-traces” (D; upper four traces in each of the four panels, labeled L_tr, L_sus, D_tr, and D_sus). Summation of each cone’s four sub-traces yields that cone’s total contribution to the cluster (D, bottom traces, labeled “sum”). Finally, summation of the four cone totals yields the full reconstruction (E, black trace), shown superimposed on the target cluster mean (gray).

(F) As (A)–(E) but showing only the weights (top), cone totals (middle), and full reconstructions (bottom) for another four example clusters (from left: C₁; C₁₅; C₁₄; and C₂₅).

Further detail on reconstructions is shown in Figure S2, and all clusters’ individual results are detailed in Data S1.