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Fig. 4.
AT7519 is a more selective CDK9 inhibitor than FVP in zebrafish. (A) Brightfield images of a cdk9+/+ (top), cdk9+/− (middle) and cdk9−/− (bottom) whole zebrafish at 4 dpf. Scale bars: 1 mm. (B) Restriction enzyme digest gel displaying cdk9 genotypes of zebrafish larvae. Hyperladder (HL) band and individual genotype bands (in order: cdk9−/−, cdk9+/− and cdk9+/+) are indicated. (C,E) Heart rate (beats/min) (C) and percentage survival (E) of cdk9−/− larvae at 2 hpt, 6 hpt, 12 hpt, 24 hpt and 48 hpt with 0.1%, DMSO vehicle, 1 μM AT7519 or 1 μM FVP treatment. (D,F) Heart rate (beats/min) (D) and percentage survival (F) of cdk9−/− larvae at 2 hpt, 6 hpt, 12 hpt, 24 hpt and 48 hpt with 0.3% DMSO vehicle, 50 μM AT7519 or 3 μM FVP treatment. (C,D) Error bars represent s.e.m., n=15 larvae, experimental n=3. **P<0.01, ***P<0.001, ****P<0.0001 (two-way ANOVA and Bonferroni post-hoc test for comparisons between DMSO vehicle or CDK9i treatment groups).