Fig. 2

Fig. 2 Fig. 2. ETS factor motifs are required for venous Ephb4-2 enhancer activity. A. Representative 48hpf F0 Tol2-mediated mosaic transgenic zebrafish expressing either wild type (upper panel) or ETS-motif mutated (lower panel) versions of the Ephb4-2:GFP transgene. Red box denotes region shown at high magnification on the left, red bracket indicates dorsal aorta, white bracket indicates cardinal vein. B. Table summarizing the n numbers and patterns of GFP expression in F0 Tol2-mediated transgenic zebrafish. ∗ indicates transgenic zebrafish already reported in Neal et al. (2019). Note that the total numbers of zebrafish screened is lower than reported in Neal et al. (2019), as they exclude analysis that did not record vein/arterial/isv expression patterns. C. Representative E11.5 F0 transgenic mouse embryos expressing either wild type (left panel) or ETS-motif mutated (right panels) versions of Ephb4-2:lacZ transgenes. cev ​= ​branches of cerebral venous plexus, cv ​= ​cardinal vein, isv ​= ​intersomitic vessel, nt ​= ​neural tube. All additional transgenic embryos are shown in Fig. S5. D. Table summarizing the n numbers and patterns of X-gal staining in F0 transgenic mouse embryos. ∗ denotes data initially reported in Neal et al. (2019).