ZFIN Image: Ear et al., 2021, Figure 2

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Figure Caption

Figure 2

GIV-L, a human transcript for GIV, translates a variant protein that contains a PBM.A, schematic of GIV and GIV-L transcript as annotated in ensembl. Indicated in red is region where transcript sequence of GIV (Exon 31) and GIV-L diverges. The panel below shows nucleotide sequence and translated sequence of indicated region. B, MeRIP-Seq analysis of GIV transcript as annotated in MeT-DB (http://compgenomics.utsa.edu/methylation/) to map degree of m6A-methylated RNA. Highlighted in red is the corresponding region of GIV-L transcript (the intron immediately downstream of exon 31) indicating the methylated region. C, reverse-transcription PCR of GIV and GIV-L transcript in multiple cell lines: DLD1 E-type and R-type (top), HCT116 (middle, left), Caco-2 (middle, right), and HeLa (bottom).

Acknowledgments

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