IMAGE

Figure 6

ID
ZDB-IMAGE-210902-287
Source
Figures for Molagoda et al., 2021
Image
Figure Caption

Figure 6

Transient knockdown of p62 aggravates the fisetin-induced stabilization of mitochondrial membrane potential. BV2 microglial cells were transiently transfected with p62 siRNA (sip62) for 48 h and subsequently treated with 1 µg/mL LPS for 2 h, followed by stimulation with 1 mM ATP (LPS/ATP) for 2 h. (A) The population of cells with depolarized mitochondrial membrane potential was measured using a Muse MitoPotential Kit. The total population of live cells with depolarized mitochondrial membrane potential (bottom left) and cell viability (bottom right) is depicted. (B) In a parallel experiment, cells were stained using 2 µM MitoSOX Red. Images of cells were captured using a CELENA S Digital Imaging System. (C) Extracellular level of IL-1β were quantified using ELISA after 48 h of stimulation of LPS/ATP. The results indicate the mean ± standard error median (SEM), and is representative of the results obtained from three independent experiments.* p < 0.05.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxidants (Basel)