Figure 6

Figure 6

Cell proliferation/viability assay in the presence of growth factors. HUVEC cells were cultured for 50 hours on collagen type 1 in medium containing 2% fetal bovine serum (FBS) and 20 ng/mL FGF-2 or 50 ng/mL VEGF-A, in the presence or absence of the HS-binding peptide. (A) VEGF-A + 2% SFB. (B) FGF-2 + 2% SFB. (C) 2% SFB. Control, HUVEC cells in the absence of treatment. Peptide, HUVEC cells with HS-binding peptide treatment (10 µM). Assay performed in triplicate. The values indicate the mean and standard deviation of the cell index (index proportional to the number of cells adhered to the plate). Peptide inhibited FGF-2 proliferation. Each point of FGF-2 assay was statically significant p<0.05 (Kruskall-Wallis) while the decrease of proliferation/viability with VEGF-A was not significant.