ZFIN Image: Melo et al., 2021, Figure 5

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Figure 5

Ex vivo angiogenesis assay. Sixteen rings of mouse aortas were collected and cultured in culture plates containing Matrigel for seven days, (A) aorta ring cultured in the absence of peptide (control); aorta ring cultured in the presence of HS-binding peptide (10 µM or 100 µM) and aorta ring cultured in the presence of scrambled peptide (10 µM). (B) The area of the formed blood vessels was analyzed using ImageJ software. The values represent means and standard deviations the area reached by the blood vessels formed from the aorta. *p < 0.05 (ANOVA). The HS-binding peptide decreased blood formation from the aorta.

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