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Figure 5 Treatment of zebrafish larvae and adult with a PRC2 inhibitor, UNC6852. (A) Comparison of time to occlusion of the caudal vein after laser injury between wild-type control 5 dpf larvae and UNC6852-injected larvae by one-way ANOVA. The x-axis represents four different concentrations of the UNC6852 (0.075 mM, 0.15 mM, 0.3 mM and 0.6 mM) used for larval injections. The dot plots represent wild-type larvae (closed circles) and the knockdown larvae (closed triangles). Error bars represent mean ± SD. 50 larvae each were used in control and UNC6852 injections (N = 50); (B) Quantitative real-time PCR showing the fold change of tfpia gene expression in liver and spleen for UNC6852-injected adult zebrafish with wild-type control by Student’s t test. The concentration of UNC6852 used for adult injections (0.6 mM) is indicated beneath the bar graph. Error bars represent mean ± SD. Six fish were used for each of the simultaneous 5-gene knockdown and control experiments (N = 6). *** and **** represent p ≤ 0.001 and p ≤ 0.0001, respectively.