Figure 1

Figure 1

Levels of tfpia mRNA in a primary knockdown screen of chromatin binding and regulatory proteins. Quantitative real-time PCR showing the fold change of tfpia gene expression in liver and spleen in 23 sets (Set 1–Set 23) of chromatin binding and regulatory protein-knockdowns compared to daily wild-type (WT) controls using one-way ANOVA. The bar graphs represent daily wild-type controls (squared bars), and all knockdowns (open bars and dotted bars) to the right of each wild-type were performed on the same day. Error bars represent mean ± SD. Six fish were used for each set of chromatin binding and regulatory protein-knockdown and control experiments (N = 6). The lines on the top represent a significant difference between wild-type and knockdown sample sets. *** and **** represent p ≤ 0.001 and p ≤ 0.0001, respectively. p-value < 0.05 was considered significant.