FIGURE 7

FIGURE 7

Examination of the functional role of TcpAh in preventing TRIF–TRAF3 and TRAF3–TBK1 interactions. (A,B) Activation of human IRF3 promoter in HEK293T cells transfected with human IRF3 luciferase reporter (HsIRF3-Luc; 200 ng/mL), renilla luciferase reporter (15 ng/mL), and (A) zebrafish TRAF3 expression vector (50 ng/mL) alone or in combination with zebrafish TRIF expression vector (50 ng/mL) with or without TcpAh expression vector (500 ng/mL) or (B) zebrafish TBK1 expression vector (50 ng/mL) alone or in combination with a zebrafish TRAF3 expression vector (50 ng/mL) with or without TcpAh expression vector (500 ng/mL). Data are the average luciferase activity ± SD (*p < 0.05; **p < 0.01; ns, not significant). (C–E) Co-IP analysis showing the inhibition of TRIF–TRAF3 and TRAF3–TBK1 interactions by TcpAh in HEK293T cells transfected with plasmids in indicated combinations.