IMAGE

Figure 1 - figure supplement 1

ID
ZDB-IMAGE-210607-30
Source
Figures for Li et al., 2021
Image
Figure Caption

Figure 1 - figure supplement 1 <italic toggle='yes'>ccm2</italic> CRISPR zebrafish exhibit mosaic expression of CCM2.

(A) Outline of the experiment: cas9 mRNA and gRNAs targeting ccm2 were co-injected into one-cell stage zebrafish embryos. (B) Sequencing showed that the Cas9 together with designed gRNA could successfully target ccm2 and produce indels. PAM sequence (5’-AGG-3’) is indicated in a rectangle. Though the wild-type sequence is the top read in each downstream position, indicating existence of wild-type allele, multiple reads emerged downstream of the PAM in ccm2 CRISPR embryos. (C) Whole mount in situ hybridization of 2 days post fertilization (dpf) embryos showed ccm2 CRISPR embryos displaying patchy loss of CCM2 expression.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife