|
Fig. 4 Expression of solute carriers (SLCs) in the pronephric duct of the developing zebrafish pronephros. A: 10 most abundant SLCs in the pronephric duct at 1 day postfertilization (dpf) (PD1) compared with the relative expression of these top 10 SLCs in the proximal convoluted tubule (PC1), proximal straight tubule (PS1), distal early tubule (DE1), and distal late tubule (DL1) as well as their expression at 2 dpf (PC2, PS2, DE2, DL2, and PD2, respectively) and 3 dpf (PC3, PS3, DE3, DL3, and PD3, respectively). B: 10 most abundant SLCs in PD3 compared with the relative expression of these top 10 SLCs in PC3, PS3, DE3, and DL3 as well as their expression at 1 dpf and 2 dpf. C: relative change in expression between 1 dpf and 3 dpf for the top 10 candidates identified in the pronephric duct at 1 dpf and 3 dpf. Green labeled bars indicate ≥60% abundance at 1 dpf; red labeled bars indicate ≥60% abundance at 3 dpf. The light green and red bars correspond to <60% abundance. D: comparison of the top 40 genes expressed 3 dpf in the proximal convoluted tubule and pronephric duct. Genes were sorted for PC3; overlapping genes were only represented once. The comparison revealed high expression of selenoprotein P (sepp1a) and megalin (lrp2) in the proximal segments, whereas proteins involved in apoptosis (atg16l1) and vesicular transport (vstm2l and cplx4b) were expressed at higher levels in the distal pronephros. PC3; pronephric cells.