ZFIN Image: Magnani et al., 2021, Figure 1

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Figure Caption

Figure 1

uhrf1 and dnmt1 loss causes DNA methylation loss in zebrafish livers. (A) Slot blot of genomic DNA extracted from pools of 5 dpf uhrf1^−/− and dnmt1^−/− mutant and WT siblings livers. (B) Quantification of 5-MeC measured by slot blot normalized to double stranded DNA (dsDNA). Each dot represents one clutch. *p < 0.05 and **p < 0.005 calculated by unpaired t-test. (C) Time course imaging of live larvae by fluorescent stereoscope microscopy. Tg(c269°ff ; 10XUAS:dsRed; fabp10a:GAL4; cmlc2:EGFP);dnmt1−/− and Tg(c269°ff; 10XUAS:dsRed; fabp10a:GAL4; cmlc2:EGFP);uhrf1−/− are shown at each age, showing the liver (dsRed), the heart (GFP) as a marker of transgenesis and, in green, the liver with DNA hypomethylation in mutants only. Images are representative of 100% of larvae from 3 clutches observed for each genotype.

Acknowledgments

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