ZFIN Image: Ambrosini et al., 2020, Figure 2

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Figure Caption

Figure 2 Long-term culture of cancer stem cells (CSCs) favors the entrance in a quiescent state. (A) Proliferation rate of Panc1 P and CSCs cultured in their specific-medium for 2, 4, 7, and 10 days. 5 × 10³ cells/well have been plated for each condition at time 0. Legend: White squared: P cells; gray rhombus: CSCs 2 weeks; black triangle: CSCs 4 weeks; star: CSCs 8 weeks. (B) Representative histograms of cell cycle analysis for each condition. (C) Cell cycle distribution of Panc1 P cells and CSCs through flow cytometry. (D) qPCR analysis of the quiescent markers MAD2L1, Cyclin B1, and RPLP0 in Panc1 P cells and CSCs. The values are reported as fold change relative to P cells. Histograms legends: white: P cells; light gray: CSCs 2 weeks; dark grey: CSCs 4 weeks; black: CSCs 8 weeks. Values are the means (± SE) of at least three independent biological replicates. Statistical legend: p < 0.05 (*) P cells versus CSCs; ($) CSCs 2 weeks versus CSCs 8 weeks; (#) CSCs 4 weeks versus CSCs 8 weeks.

Acknowledgments

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