Figure 4

Figure 4

Development of nonalcoholic steatohepatitis (NASH) phenotypes in LmiR-21 zebrafish at 6 mpf. (A) Gross liver images of (a) LmiR21 − Dox male, (b) LmiR21 + Dox male, (c) LmiR21 − Dox female, (d) LmiR21 + Dox female. Scale bar: 3 mm. (B) Representative images of Masson’s trichrome staining of steatohepatitis in livers of (1) LmiR21 − Dox (insets a-d) and (2) LmiR21 + Dox; Insets: (a) hepatocyte ballooning, (b) Mallory-Denk bodies (MDBs) are indicated by arrows, and (c,d) lobular inflammation is indicated by arrows. Scale bar: 50 μm. (C) Representative images of Masson’s trichrome staining of fibrotic liver tissue from LmiR21 + Dox; Insets: (a,b) MDBs concomitant with fibrosis (red arrows) and scar tissue associated with liver fibrosis (yellow arrows) and (c,d) lobular inflammation concomitant with blue collagen (blue arrows). Scale bar: 50 μm. (D) Induction of proinflammatory gene expressions in response to inflammation was significantly greater in LmiR21 + Dox than in controls (n = 3). Statistically significant differences from LmiR21 − Dox were denoted by * (p < 0.05), ** (p < 0.01).