ZFIN Image: Yang et al., 2021, Figure 2

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Figure Caption

Figure 2 Determination of cell viability by the MTT assay in the myotubes. (a) Myotubes were incubated with the indicated concentrations of DPHC for 24 h. (b) Myotubes were starved in serum-free media and incubated for 24 h with DPHC (3, 10, and 30 μM) and 2-NBDG (50 μM). Detection of cytosolic Ca²⁺ levels using the Fluo-4 calcium indicator in the myotubes. Myotubes were loaded with Fluo-4 in PSS in the (c) presence or (d) absence of Ca²⁺ and treated with control (PSS only), 30 μM of DPHC, or BAPTA-AM. Box plots representation of (e) the cytosolic Ca²⁺ levels in myotube responses after 30 μM of DPHC or BAPTA-AM treatment as presented in (c) and (d). Data are expressed as the mean± SE , n = 3 per group, *p<0.05 compared with the nontreated group. N.S.: no significance compared with the DPHC in the presence or absence of extracellular Ca²⁺.

Acknowledgments

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